Evaluation Of Antimicrobial Effects Of Orthodontic Band Cement Incorporated With Zirconia, Gold, Copper Biosynthesized Nanoparticles- An In-Vitro Study Original Research
Main Article Content
Abstract
OBJECTIVE: To evaluate the antimicrobial effects of orthodontic band cement incorporated with various biosynthesized nanoparticles.
MATERIALS AND METHODS: Zirconia, gold, copper Nanoparticles were green synthesized using white tea extract and dry ginger which are incorporated into orthodontic band cement, characterized using FTIR analysis. Antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus was tested in vitro by disc diffusion and Time Kill Curve Assay.
RESULTS: Zirconia nanoparticles incorporated GIC exhibited better antimicrobial activity against streptococcus mutans, and gold nanoparticles incorporated GIC against lactobacillus acidophilus at varying concentrations at different time period.
CONCLUSION: The antibacterial effect against streptococcus mutans and lactobacillus acidophilus were distinctly noted in Zirconia nanoparticles, gold nanoparticles followed by copper nanoparticles. Zirconia and gold nanoparticles produced more efficient antibacterial property and eventually would be effective in curtailing White Spot Lesions.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Harrison, J., 2011. Orthodontic treatment. Vital, 8(1), pp.31-35.
Godoy F et al, Prevalence of banding and bonding molar brackets in orthognathic surgery cases. J Oral Maxillofac Surg. 2011; 69(3): 911-16.
Brown D. Orthodontic band cements. Br J Orthod. 1989;16(2):127-31.
Tufekci E et al, Prevalence of white spot lesions during orthodontic treatment with fixed appliances. Angle Orthod. 2011;81(2):206-210.
Lundström F et al, Streptococcus mutans and lactobacilli frequency in orthodontic patients; the effect of chlorhexidine treatments. Eur J Orthod. 1987;9(2):109-116.
Borzabadi-Farahani A et al, Nanoparticles in orthodontics, a review of antimicrobial and anti-caries applications. Acta Odontol Scand. 2014;72(6):413-417.
Nam NH et al, Nanoparticles: synthesis and applications. Materials for Biomedical Engineering. 2019;211-240.
Moreira DM, Oei J, Rawls HR, et al. A novel antimicrobial orthodontic band cement within situ-generated silver nanoparticles. Angle Orthod. 2015;85(2):175-183. doi:1
Yi J et al, A nano-CaF2-containing orthodontic cement with antibacterial and remineralization capabilities to combat enamel white spot lesions. J Dent. 2019; 89:103172.
Robertson MA et al, MI Paste Plus to prevent demineralization in orthodontic patients: a prospective randomized controlled trial. Am J Orthod Dentofacial Orthop. 2011;140(5):660-668.
Spencer CG et al, Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod. 2009;79(2):317-322.
Farret MM et al, Can we add chlorhexidine into glass ionomer cements for band cementation? Angle Orthod. 2011;81(3):496-502.
Rajendran K et al, Toxicological evaluation of biosynthesized hematite nanoparticles in vivo. Colloids Surf B Biointerfaces. 2021; 198:111475.
Londhe SM et al, Efficacy of Second Molar to Achieve Anchorage Control in Maximum Anchorage Cases. Med J Armed Forces India. 2010;66(3):220-224.
Mizrahi E. Success and failure of banding and bonding. A clinical study. Angle Orthod. 1982;52(2):113-117.
Millett, D.T., Glenny, A.M., Mattick, R.C., Hickman, J. and Mandall, N.A., 2016. Adhesives for fixed orthodontic bands. Cochrane Database of Systematic Reviews, (10).
Ma, X., Zhou, S., Xu, X. and Du, Q., 2022. Copper-containing nanoparticles: Mechanism of antimicrobial effect and application in dentistry-a narrative review. Frontiers in Surgery, 9, p.905892.
Rocha ACDS et al, Core-shell nanoparticles based on zirconia covered with silver as an advantageous perspective for obtaining antimicrobial nanocomposites with good mechanical properties and less cytotoxicity. J Mech Behav Biomed Mater. 2021; 123:104726.
Brown ML et al, Ion release from a novel orthodontic resin bonding agent for the reduction and/or prevention of white spot lesions. An in vitro study. Angle Orthod. 2011;81(6):1014-1020.
Haghparasti Z et al, Green synthesis of water-soluble nontoxic inorganic polymer nanocomposites containing silver nanoparticles using white tea extract and assessment of their in vitro antioxidant and cytotoxicity activities. Mater Sci Eng C Mater Biol Appl. 2018; 87:139-148.
Janaki AC et al, Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc. 2015; 144:17-22.
Bhat DV et al, Evaluation of Remineralizing Potential of CPP-ACP, CPP-ACP + F and β TCP + F and Their Effect on Microhardness of Enamel Using Vickers Microhardness Test: An In Vitro Study. Int J Clin Pediatr Dent. 2022;15(Suppl 2): S221-S225.
Freitas Jr, R.A., 2000. Nanodentistry. The Journal of the American Dental Association, 131(11), pp.1559-65.
Goldberg M et al, In vitro and in vivo studies on the toxicity of dental resin components: a review. Clin Oral Investig. 2008;12(1):1-8.
Song JH, Kim SK, Chang KW, Han SK, Yi HK, Jeon JG. In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. Arch Oral Biol. 2006.